Production of biopharmaceutical of fructo-oligosaccharide for promoting bacterial gut-microbiota


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Start date01/10/2023

End date30/09/2024


Abstract

Prebiotic dietary fibers called fructo-oligosaccharides are good for your health. Since it promotes the growth of beneficial microbes in the gastrointestinal system while preventing the growth of harmful microorganisms, it has been approved by the Food and Drug Administration of Japan, the United States, and the European Union for more than 20 years. It controls digestive tract motility, promotes digestion, and increases excretion, avoiding constipation and lowering the risk of colon cancer. Fructo-oligosaccharides also influence how fat and glucose are metabolized, leading them to maintain healthy levels of lipids and blood sugar. As a result, it is frequently utilized in dietary supplements for those who want to regulate their blood sugar levels or weight, as well as for intestinal cleansing.

There are two methods for producing fructo-oligosaccharides biologically. The first way is the extraction of long-chain fructo-oligosaccharides (more than 4 units in length) from inulin-rich plants by the endo-inulinase enzyme. The second method involves the synthesis of short-chain fructo-oligosaccharides (3–4 unit in length) from sucrose using fructosyltransferase and beta-fructo-furanosides (Invertase). Kestose is a fructo-oligosaccharide (trimer) that has more bioactive and prebiotic properties than long-chain fructo-oligosaccharides. Therefore, this research project is interested in the selection, study, and production of invertase enzymes for the synthesis of kestose. The production of fructo-oligosaccharide will be scaled-up in laboratory-scale bioreactors. The efficacy of fructo-oligosaccharides in fostering intestinal microbial communities will be evaluated. Additionally, sucrose is the most common sugar in agricultural and industrial waste, such as molasses. Therefore, the information learned from the project can be applied to this waste in the future.


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Last updated on 2025-03-10 at 11:18