Single laboratory method validation for antibody conjugated silica nanoparticles for rapid detection of Campylobacter jejuni

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Publication Details

Author listPiyatida Sutnut, Supakorn Pansakul, Peerapon Chaisalee, Pravate Tuitemwong, and Kooranee Tuitemwong

Publication year2022


Abstract

This research was to perform single laboratory method validation for antibody conjugated silica nanoparticles (FDS-NPs) for the rapid detection of Campylobacter jejuni in chicken samples. The FDS-NPs have a round shape with an average size of 10-100 nm. Some aggregates were detected. FT-Raman spectrum revealed that FDS-NPs surface was modified with carboxyl functional group and allowed them to bind with monoclonal antibodies against C. jejuni. The antibody-conjugated FDS-NPs attached to bacteria cell and could be identified clearly under epifluorescent microscope. The cells were extremely bright orange with rounded surface and moved directionless under microscope. FDS-NPs were used to detect C. jejuni in chicken samples from a slaughterhouse (100 carcass rinsed, 100 cloacal swab and 100 viscera content) according to ISO16140 guidance. Values of relative accuracy, specificity, and sensitivity for FDS-NPs were 85.0, 82.5 and 90.0%, respectively. The Kappa value of test of inter-agreement was acceptable, compared with standard method. FDS-NPs technique was equivalent to conventional but gave much faster results (30 min compared with 5 days).


Keywords

Campylobacter jejuni


Last updated on 2022-17-08 at 23:05