Isolation and characterization of probiotics from pig manure on multi-drug resistant pathogens
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Publication Details
Author list: Chalida Neamwadee, Peerapon Chaisalee, Parichut Rattanapol, Piyarat Khanthawaro and Kooranee Tuitemwong
Publication year: 2022
Start page: 91
Abstract
The objectives of this study were to evaluate effectiveness of probiotics (lactic acid bacteria, LAB; and Bifidobacterium spp.) on some foodborne pathogens (Salmonella spp.) in porcine caeca samples after 30 days administration. It was found that numbers of LAB and Bifidobacterium spp. were high in the range of Log 8-Log 9 cfu/g and Log 8-Log 9 cfu/g, respectively. Numbers of pathogenic Salmonella spp. were in the low range from not detected (less than 10 cfu/g) to Log 2 cfu/g. However, Escherichia coli numbers were quite stable at high counts of Log 6-Log 7 cfu/g. The normal flora E. coli numbers remained normal after probiotic intervention. Biochemical characteristics of isolates indicated that 20 LAB isolates felt into 2 strains (Lactobacillus acidophilus and Lactobacillus fermentum), 10 isolates of Bifidobacterium spp. fell into 2 strains (B1, B2). Fourteen (14) Salmonella isolates were assigned to 1 serovars (Sal-2). Salmonella strains were resistant to kanamycin, deoxycycline, amoxicillin, amplicilin and penicillin. Salmonella strains were sensitive to choloramphenical. We have concluded that probiotics could sustain at high count in porcine caeca and suppressed pathogenic Salmonella into very low counts after 1 month of the administration. All Salmonella isolates were multi-drug resistant strains. It was evident that the probiotics could be preferable choice to control animal pathogens without using antibiotics.
Keywords
Fecal Coliform, Muti-drug, Probiotic, Salmonella