Distance-based paper device using combined SYBR safe and gold nanoparticle probe LAMP assay to detect Leishmania among patients with HIV

Journal article

Authors/Editors

WIJITAR DUNGCHAI

Strategic Research Themes

Medical Treatment & Prevention (Smart Healthcare)

Publication Details

Author list: Toon Ruang-areerate, Natkrittaya Saengsawang, Panthita Ruang-areerate, Nalin Ratnarathorn, Thanyapit Thita, Saovanee Leelayoova, Suradej Siripattanapipong, Kiattawee Choowongkomon and Wijitar Dungchai

Publisher: Nature Research

Publication year: 2022

Volume number: 12

Issue number: 1

ISSN: 2045-2322

eISSN: 2045-2322

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85136873960&doi=10.1038%2fs41598-022-18765-w&partnerID=40&md5=fd122bf6558725a3421454487b29fa4d

Languages: English-Great Britain (EN-GB)

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Abstract

Asymptomatic visceral leishmaniasis cases increase continuously, particularly among patients with HIV who are at risk to develop further symptoms of leishmaniasis. A simple, sensitive and reliable diagnosis is crucially needed due to risk populations mostly residing in rural communities with limited resources of laboratory equipment. In this study, a highly sensitive and selective determination of Leishmania among asymptomatic patients with Leishmania/HIV co-infection was achieved to simultaneously interpret and semi-quantify using colorimetric precipitates (gold-nanoparticle probe; AuNP-probe) and fluorescence (SYBR safe dye and distance-based paper device; dPAD) in one-step loop-mediated isothermal amplification (LAMP) assay. The sensitivities and specificities of 3 detection methods were equivalent and had reliable performances achieving as high as 95.5%. Detection limits were 102 parasites/mL (0.0147 ng/µL) which were 10 times more sensitive than other related studies. To empower leishmaniasis surveillance as well as prevention and control, this dPAD combined with SYBR safe and gold nanoparticle probe LAMP assay is reliably fast, simple, inexpensive and practical for field diagnostics to point-of-care settings in resource-limited areas which can be set up in all levels of healthcare facilities, especially in low to middle income countries. © 2022, The Author(s).

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