Highly sensitive and selective antibody microarrays based on a Cy5-antibody complexes coupling ES-biochip for E. coli and Salmonella detection

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Hormsombut, Timpika; Rijiravanich, Patsamon; Surareungchai, Werasak; Kalasin, Surachate;

ผู้เผยแพร่: Royal Society of Chemistry

ปีที่เผยแพร่ (ค.ศ.): 2022

วารสาร: RSC Advances (2046-2069)

Volume number: 12

Issue number: 38

หน้าแรก: 24760

หน้าสุดท้าย: 24768

จำนวนหน้า: 9

นอก: 2046-2069

eISSN: 2046-2069

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138472103&doi=10.1039%2fd2ra03391g&partnerID=40&md5=1a5dd748f8f28de8131f06b60b49da4d

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Foodborne pathogens are threats in food and a cause of major health issues globally. Microbial safety has become a key concern to eliminate disease-causing pathogens from the food supply. For this purpose, the Cy5 dye conjugated with a double-biotin DNA linkage and a detection antibody (Cy5-Ab complexes) was developed to amplify a foodborne detection signal on a microarray. Additionally, the ES-biochip was designed to attain a visual screening of an antibody microarray for the simultaneous threat detection of Salmonella and Escherichia coli (E. coli). Quantification was also performed by fluorescence. After optimizing the Cy5-Ab complex appendage and enhancing the detection signal from a sandwich immunoassay, high sensitivity and selectivity were observed. The limits of detection for both pathogens in buffer and food samples were 103 CFU mL−1 and less than 9 CFU mL−1 by visual screening and fluorescent intensity quantification, respectively. Mono and duplex responses were not significantly different which means that no cross-reactivity occurred. Uniquely, the assays hold great potential to be used in several fields, such as clinical diagnosis of foodborne microbes, food hygiene screening, and pathogen detection. © 2022 The Royal Society of Chemistry.

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