Proteomic Profile of novel Saccharomyces cerevisiae RSO4 Strain on Hom Mali Rice Fermentation

Proteomic analysis is one of the most powerful approaches for understanding the expression profiles of molecular systems. This study shows, we presented the physiological and proteomic expression profiles of a newly identified Saccharomyces cerevisiae RSO4 strain that cover several features of its fermentation performance and the proportion of all proteins expressed during the fermentation of Hom Mali rice, Oryza sativa. The yeast RSO4 strain displayed a distinct expression profile, as well as increased tolerance to high concentrations of sugar and the ability to grow well on alternative carbon sources. Functional analysis of the proteome was conducted using Kyoyo Encyclopedia of Genes and Genomes (KEGG), the Carbohydrate-Active Enyme (CAZy) and the Saccharomyces Genome database (SGD) revealed extensive alteration in the cellular metabolisms, including the glycolysis/gluconeogenesis pathways as well as the starch and sucrose sugar metabolism. Their gene products were differentially expressed in high abundance. Some biotechnologically important enzymes such as β-glucosidases and glycogen-branching enzymes were retrieved from the proteomic database. Thus, owing to its enhanced fermentation performance, the yeast RSO4 strain could be an ideal candidate for functional food applications using the starch-based media culture.