Ethyl Acetate Extract of Halymenia durvillei Induced Apoptosis, Autophagy, and Cell Cycle Arrest in Colorectal Cancer Cells

Journal article

Authors/Editors

NAKORN NIAMNONT

Strategic Research Themes

Sustainable Bioeconomy (Strategic Research Themes)

Publication Details

Author list: Chantree, Pathanin; Martviset, Pongsakorn; Sornchuer, Phornphan; Thongsepee, Nattaya; Sangpairoj, Kant; Meemon, Krai; Niamnont, Nakorn; Tamtin, Montakan; Sobhon, Prasert;

Publisher: Korean Society of Food Science and Nutrition

Publication year: 2023

Volume number: 28

Issue number: 1

Start page: 69

End page: 78

Number of pages: 10

ISSN: 2287-1098

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85159348202&doi=10.3746%2fpnf.2023.28.1.69&partnerID=40&md5=7c56e9fb85bc05a749d450e3b86f31d9

Languages: English-Great Britain (EN-GB)

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Abstract

Colorectal cancer is one of the most death-dealing cancers. However, conventional cancer treatments still have side effects. Therefore, novel chemotherapeutic agents with less side effects are still in search. A marine red seaweed, Halymenia durvillei, is recently interested in its anticancer effects. This study investigated the anticancer effect of ethyl acetate extract of H. durvillei (HDEA) on HT-29 colorectal cancer cells in association with the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway. HDEA-treated HT-29 and OUMS-36 cells were used for cell viability tests by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide assay. The effects of HDEA on apoptosis and cell cycle were evaluated. The nuclear morphology and mitochondrial membrane potential (∆ψm) were observed by Hoechst 33342 and JC-1 staining, respectively. The gene expression of PI3K, AKT, and mTOR genes was evaluated using a real-time semiquantitative reverse transcription-polymerase chain reaction. The corresponding protein expressions were assessed by western blot analysis. The result revealed that the cell viability of treated HT-29 cells diminished while that of OUMS-36 cells was non-significant. By the down-regulation of cyclin-dependent kinase 4 and cyclin D1, HDEA-treated HT-29 cells were arrested in the G0/G1 phase. By the up-regulation of cleaved poly(adenosine diphosphate-ribose) polymerase, caspase-9, caspase-8, caspase-3, and Bax, HDEA-treated HT-29 cells underwent apoptosis, but suppressed Bcl-2, disrupted nuclear morphology and ∆ψm. Furthermore, treated HT-29 cells underwent autophagy by up-regulation of light chain 3-II and beclin-1. Lastly, HDEA suppressed the expression of PI3K, AKT, and mTOR. Therefore, HDEA exerts anticancer effects against HT-29 cells, confirmed by apoptosis, autophagy, and cell cycle arrest induction via regulation of the PI3K/AKT/mTOR signaling pathway. © 2023 The Korean Society of Food Science and Nutrition.

Keywords

H. durvillei