Proteomic Analysis during the Production of Xylolipid Biosurfactant in the Yeast Meyerozyma and the effect on the Biofilm Formation

Biosurfactants have been shown to have a various application in medicines. Antibiofilm activity is a desirable property of biosurfactants. Several biosurfactants produced from bacteria have antibacterial activity against pathogens, making them a promising biocontrol molecule for ensuring medical sustainability in the long run. The purpose of this research was to characterize biosurfactant produced by the yeast Meyerozyma species and the effect of salt and palm oil on biosurfactant production. Maximum xylolipid production was the higest concentration at approximately 30 g/L. Oil displacement equaling nearly 10.0 cm and reduction in surface tension was more than 40 mN/m obtained under the condition of high levels of palm oil salts. Protein-protein association tool indentified tripled expression of proteins associated to lipid metabolism. Interestingly, xylolipid could inhibit biofilm formation against the opportunistic yeast Candida albicans after 2 h-treatment. Xylose induced the coordinated expression of key genes, involved in hyphal, adhesion, cell cycle and cell morphology shown by gene expression analysis. Overall, the biosurfactant-producing strains identified under this investigation show promising property for future development and potential use in the cost-effective industrial-scale production using yeast as a cell factory in biotechnological processes.