Pyrene-Labeled and Quaternized Chitosan: Synthesis, Characterization, and Its Potential Application for Fluorescently Trackable Nucleic Acid Delivery into Cells

Journal article

Authors/Editors

Strategic Research Themes

Publication Details

Author list: Pratumyot, Kornkanya; Yuntasiri, Pongsakorn; Khunsuk, Phim-On; Phuangkaew, Tinnakorn; Sittplangkoon, Chutamath; Pattarakankul, Thitiporn; Palaga, Tanapat; Kiatkamjornwong, Suda; Hoven, Voravee P.

Publisher: American Chemical Society

Publication year: 2023

Journal acronym: Biomacromolecules

Volume number: 24

Issue number: 9

Start page: 4005

End page: 4018

Number of pages: 14

ISSN: 1525-7797

eISSN: 1526-4602

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85168572880&doi=10.1021%2facs.biomac.3c00301&partnerID=40&md5=5755362df2b28fbb9587a3557b2d3e0f

Languages: English-Great Britain (EN-GB)

View on publisher site

Abstract

A chitosan derivative (Pyr-CS-HTAP) having pyrene (Pyr) and N-[(2-hydroxyl-3-trimethylammonium)] propyl (HTAP) units conjugated at C6 and C2 positions, respectively, was synthesized and characterized. Dynamic light scattering and scanning electron microscopy revealed that Pyr-CS-HTAP self-assembled into spherical nanoparticles with a hydrodynamic diameter of 211 ± 5 nm and a ζ-potential of +49 mV. The successful binding of Pyr-CS-HTAP with nucleic acid was ascertained by fluorescence resonance energy-transfer analysis and gel electrophoresis. Pyr-CS-HTAP facilitated the cellular uptake of nucleic acid up to 99%. Co-localization analysis using fluorescence microscopy revealed the endosomal escape of the Pyr-CS-HTAP/nucleic acid complexes and the successful release of the nucleic acid cargoes from the polyplexes into the nucleus. It is strongly believed that Pyr-CS-HTAP can potentially be developed into a fluorescently trackable gene delivery system in the future. © 2023 American Chemical Society.

Keywords

No matching items found.