A strategy for axenic cultures of Arthrospira sp. with cycloheximide and berberine
Poster
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Strategic Research Themes
Publication Details
Author list: Nareerat Peepim, Kalyanee Paithoonrangsarid, Wipawan Siangdung, Wattana Jeamton
Publication year: 2023
Title of series: T H E ANNUAL M EETING OF T H E T H AI SOCIETY FOR BIOTEC HNOLOG Y AND INTERNATIONAL CONFERENCE
Start page: 286
End page: 286
Number of pages: 1
URL: https://tsb2023.sut.ac.th/
Abstract
Axenic culture of cyanobacteria is essential for basic and applied research in biotechnology applications. Contamination with various microbial organisms, such as fungi and bacteria, is commonly found in cyanobacteria cultures isolated from natural environments. In addition, contamination might occur during research and culture maintenance in a laboratory. In this study, we developed a strategy to obtain the axenic cyanobacterium Arthrospira platensis using strain BP as a model organism. Multiple antimicrobials were applied to eliminate contamination in the culture. Based on the results, we proposed a strategy for obtaining an axenic culture. In the first step, the addition of 200 µg mL-1 of cycloheximide to Arthrospira culture within 24 hours effectively eliminated fungal growth. Subsequently, the fungal-free culture was treated with berberine, a natural antibacterial agent from plants, at a concentration of 10 µg mL-1 for 6 hours. This treatment inhibited growth and reduced the number of most contaminated bacterial species in Arthrospira culture, including the gram-negative bacterium Arthrospiribacter sp. Finally, an axenic Arthrospira cell culture was obtained by transferring a single filament of the decontaminated cell culture grown on an agar medium containing 5% glucose and 0.5% casamino acid to the new medium. Therefore, this strategy can be further applied to other Arthrospira species.
Keywords
Antimicrobial, Arthrospira, cyanobacteria
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