Heat stress response of Arthrospira platensis C1 in the presence of urea

Poster


ผู้เขียน/บรรณาธิการ


กลุ่มสาขาการวิจัยเชิงกลยุทธ์


รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง Hsu Lwin Wai, Wipawan Siangdung, Saiwaree Ngoenphoemsup, Panittra Taweesang, Kalyanee Paithoonrangsarid

ปีที่เผยแพร่ (ค.ศ.)2023

หน้าแรก182

หน้าสุดท้าย182

จำนวนหน้า1


บทคัดย่อ

Nitrogen sources play an important role in the production of protein and phycocyanin contents, which are also affected by temperature. Urea has been proposed as an alternative nitrogen source for Arthrospira platensis cultivation. To develop a low-cost medium by substituting the standard Zarrouk’s medium, containing sodium nitrate, with urea for outdoor cultivation, understanding the response of urea-grown cells to heat stress is necessary. This research aimed to elucidate the response of A. platensis C1 grown in a medium containing urea to heat stress. When the temperature was shifted from 35 °C to 42 °C, urea-grown cells exhibited fewer negative effects on growth than nitrate-grown cells. Moreover, urea-grown cells showed an interesting increase of about 13% and 40% in protein and phycocyanin contents, respectively, without changing the carbohydrate content after 24 hours of exposure to heat stress compared to cells grown at the control temperature. Furthermore, the transcriptional responses of genes related to the increase in protein and phycocyanin contents, such as genes involved in urea uptake, phycocyanin, carbon-lipid mechanisms, and transcriptional regulation of C/N homeostasis, were also increased. Thus, using urea as a nitrogen source reduced the medium cost by about 70% and assisted cells enhancing the protein and phycocyanin contents in response to heat stress.


คำสำคัญ

HeatPhycocyaninProteinTranscriptionUrea


อัพเดทล่าสุด 2024-28-02 ถึง 23:05