Validation of electrochemical device setup for detection of dual antibiotic drug release from hydrogel
บทความในวารสาร
ผู้เขียน/บรรณาธิการ
กลุ่มสาขาการวิจัยเชิงกลยุทธ์
รายละเอียดสำหรับงานพิมพ์
รายชื่อผู้แต่ง: Sukonthachat, Jutharat; Bubpamala, Theeraporn; Poo-arporn, Rungtiva P.; Pholpabu, Pitirat
ผู้เผยแพร่: Elsevier
ปีที่เผยแพร่ (ค.ศ.): 2024
วารสาร: Journal of Pharmaceutical and Biomedical Analysis (0731-7085)
Volume number: 245
หน้าแรก: 116165
นอก: 0731-7085
eISSN: 1873-264X
ภาษา: English-Great Britain (EN-GB)
บทคัดย่อ
Due to antimicrobial resistance that occurs throughout the world, antibiotic-releasing hydrogel with at least two drugs that synergistically treat stubborn bacteria is preferable for infection prevention. Hydrogel can serve as a drug reservoir to gradually release drugs in a therapeutic window to effectively treat microorganisms with minimal side effects. The study and development of drug releasing hydrogels requires a reliable, straightforward, cost-effective, fast, and low labor-intensive drug detection technique. In this study, we validate the electrochemical technique and device setup for real-time determination of dual antibacterial drugs released from a hydrogel. Concentrations of two representative antibacterial drugs, tetracycline (TC) and chloramphenicol (CAP), were determined using square wave voltammetry (SWV) mode that yields the lower limit of detection at 2.5 µM for both drugs. Measurement accuracy and repeatability were verified by 36 known drug combination concentrations. Capability in long-term measurement was confirmed by the measurement stability which was found to last for at least 72 h. Stirring was revealed as one of the significant factors for accurate real-time detection. Real-time measurement was ultimately performed to demonstrate the determination of multiple drug releases from a drug releasing hydrogel and validated by high-performance liquid chromatography (HPLC). All the results support that the electrochemical technique with the proposed device design and setup can be used to accurately and simultaneously determine dual drugs that are released from a hydrogel in real-time. © 2024 Elsevier B.V.
คำสำคัญ
Drug releasing hydrogel
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