The Use of Human Platelet Lysate as a Coating Substance for Adipose-Derived Stem Cell Expansion

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Publication Details

Author list: Tragoonlugkana, Patcharapa; Chitchongyingcharoen, Narong; Pruksapong, Chatchai; Hassan, Shirmeela;
Ngamkham, Kamolchanok; Kamprom, Witchayapon; Supokawej, Aungkura

Publisher: IMR Press

Publication year: 2024

Volume number: 29

Issue number: 2

ISSN: 2768-6701

eISSN: 2768-6698

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85186742689&doi=10.31083%2fj.fbl2902088&partnerID=40&md5=6e728f56540472282325bc48796e044a

Languages: English-Great Britain (EN-GB)

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Abstract

Background: Large-scale production of mesenchymal stromal cells is essential for sufficient therapeutic doses in regenerative medicine. However, long-term cultivation encounters limited cell growth and cellular aging. Therefore, an alternative cell culture approach that promotes proliferation and attenuates cell senescence is required. Human platelet lysate (HPL) is a potent supplement for in vitro cell expansion. Applying HPL as a coating material can potentially improve mesenchymal stromal cell cultures. Method: To examine the capacity of HPL, it was used to pre-coat a tissue culture plate for in vitro adipose-derived mesenchymal stromal cell expansion. Alterations in biological features of adipose-derived stem cells (ADSCs) were investigated, including cell adhesion assays, cell proliferation, population doubling time, and cellular senescence. Results: ADSCs cultured on HPL-coated plates significantly increased cell adhesion rate, shortened population doubling time, and stimulated cell growth. The senescent cells were significantly decreased in ADSCs cultured in an HPL-coated plate, and the expression levels of senescence-associated genes, including p16, p21, and p53, were downregulated. Furthermore, Western blotting analysis revealed that HPL was enriched with fibronectin and vitronectin, essential cell adhesive proteins. Conclusions: HPL was effectively used as a coating material for ADSC expansions. Cellular cultivation on the HPL coating is an alternative approach for producing mesenchymal stromal cells. © 2024 IMR Press Limited. All rights reserved.

Keywords

adipose-derived stem cell, cell senescence, human platelet lysate, mesenchymal stromal cell