Light-emitting plants are development by chemiluminescence: novel chemical activator (Divanillyl oxalate), optimal conditions and plant stress

Most commercial chemiluminescent materials contain chlorine derivatives, which are highly carcinogenic to living organisms and the environment. Therefore, this research aims to synthesize greener chemiluminescence from the food flavoring substance ethyl vanillin. The objective of light-emitting plants development is using synthetic chemicals with higher light-emitting efficiency and investigate the toxicity of chemiluminescent substance on the physical characteristics of plants, paving the way for further research. The novel divanillyl oxalate (DVO) was used as a precursor in a chemiluminescence system by mixing it with a fluorophore, NaOH, and H2O2, and then measuring light emissions before spraying it onto Epipremmumaureum leaves. Additionally, components of the chemiluminescence system with various types of organic solvents were sprayed onto plant leaves to observe damage.The results showed that the DVO fluorescent system could emit light up to 2.07 x 106 a.u. in the presence of 0.5 mL DVO, 0.5 mL fluorophore, and 0.2 mL H2O2without adding additional NaOH, which was the lowest concentration at which the chemiluminescent system could still be observed by the naked eye. Moreover, after plant leaves were exposed to chemiluminescent DVO, the relative light intensity unit (RLU) of plants could glow up to 1.08 x 102 and remain visible to the naked eye for up to 60 min. Additionally, dimethyl phthalate (DMP) was an organic solvent that causes minimal damage to plants.