Evaluation of HEX293.2sus growth in 5 commercial media

Human embryonic kidney 293 (HEX 293) cells are widely used as cell factories for production

of recombinant therapeutic proteins because of their stable growth and high protein production

on large scale. At present, various commercial culture media are available for HEX 293, yet

there is no report on comparative cell growth performance among these media. In this work,

we compared the growth of HEK293.2sus cells cultured in BalanCD HEK293, CDM4HEK293,

CD293, FreeStyle 293, and 293 SFM II in shake flasks. First, 3x105 cells/mL of HEX 293.2sus

cells were seeded into 20 mL of each culture medium supplemented with 4mM L-glutamine

and 0.01 %v/v anti-clumping in a 125-mL Erlenmeyer flask, and cultured at 37 °C, 5% CO2,

100 rpm for 9 days. The cultures were sampled every day over the culture period for analysis

of viable cell density, specific growth rate and %viability. The results revealed that

CDM4HEK293 was the best media for HEK293.2sus growth, providing the max cell density,

the %viability at max cell density, and the specific growth rate around 5.62´106 cells/mL,

88.73%, and 0.44 day-1, respectively.