Exploring bioluminescence in Aglaonema: Investigating Vibrio campbellii translocation and plant responses under CaCl₂ stimulation
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Author list: Dolphen R.; Treesubsuntorn C.; Kanjanapokin C.; Chonjoho N.; Anusaraporn S.; Julpanwattana P.; Praditsmanont A.
Publisher: Elsevier
Publication year: 2024
Journal acronym: Environ Res
Volume number: 257
ISSN: 0013-9351
Languages: English-Great Britain (EN-GB)
Abstract
The feasibility of creating light-emitting plants by immobilizing Vibrio campbellii RMT1 on the rhizospheric zone of Aglaonema sp. ‘Banlangngoen’ was investigated in depth, including bacteria translocation and plant response. Results from scanning electron microscope showed that an inorganic salt-containing medium affected the root. However, transmission electron microscope results displayed bacteria translocation through the root to the leaf and colonized in the cytosol of vascular tissues. Bacteria cell counts exhibited high colonization in the root zone, approximately 3.65 × 106 CFU/mL, resulting in a light-emitting intensity increase of 23.68-fold higher than the control after the first week. Nevertheless, light microscope revealed that inorganic salts in the culture medium led to enlarged air spaces, resulting in leaf and stalk withering. Notably, spraying plants with calcium chloride (CaCl2) solution effectively mitigated salt stress, activated luminescence, and facilitated bacterial movement from roots to leaves. Additionally, CaCl2 contributed to ongoing salinity reduction in the culture medium, as evidenced by reduced malondialdehyde levels, alongside increased indole-3-acetic acid and salicylic acid concentrations, indicating plant defense responses. The interaction between plants and luminescent bacteria demonstrated the potential for producing glowing plants following CaCl2 application, addressing salinity stress, enhancing luminescence, and maintaining plant growth. © 2024
Keywords
V. campbellii
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