Supplementation of Bacillus subtilis probiotics supports health and gut microbiome in barramundi (Lates calcarifer, Bloch 1790)

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กานต์ธิดา กุศลมโน

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

เศรษฐกิจฐานชีวภาพ (รูปแบบการวิจัยเชิงกลยุทธ์)

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Tanpichai P.; Kusonmano K.; Jantrakajorn S.; Piamsomboon P.; Wongtavatchai J.

ผู้เผยแพร่: Elsevier

ปีที่เผยแพร่ (ค.ศ.): 2025

วารสาร: Aquaculture (0044-8486)

Volume number: 596

Issue number: 741815

นอก: 0044-8486

eISSN: 1873-5622

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85207762510&doi=10.1016%2fj.aquaculture.2024.741815&partnerID=40&md5=90f1263b5b4eeb555e19f80b260a326e

ภาษา: English-Great Britain (EN-GB)

ดูบนเว็บไซต์ของสำนักพิมพ์

บทคัดย่อ

The present study aimed to compare the efficacy of orally and immersion-administered Bacillus subtilis as a probiotic on fish health. The study was conducted in juvenile barramundi Lates calcarifer (average body weight 10 ± 0.7 g) following 60 days of administration. The probiotics were given to the fish either as a dietary supplement (5 × 109 CFU kg−1 diet) or by immersion (3 × 109 CFU L−1 water). At the end of the trial, fish were evaluated for growth rate, cortisol levels in response to physical stress, immune-related and growth factor gene expression, gut microbial community, and survival following a 7-day Streptococcus iniae challenge. The oral and immersion groups observed an increase in average weight gain and relative growth rate compared to the control group. Serum cortisol levels were not different among the treatments. Significant upregulation of immune-related genes (MHC I, MHC II, IL-1β, and IL-10) and a growth factor gene (OCC) were observed in the intestine of the oral group compared to the control group (p < 0.05). A significant difference in the alpha diversity index of the gut microbiome was detected in the oral compared to the immersion and control groups (p < 0.05), but the beta diversity index was not different among the three groups (p > 0.05). Both probiotic groups increased the abundance of genus Nautella, Lactobacillus, Rubritalea, and Ruegeria, while Limimaricola and Bacteroides were abundant only in the oral group. Despite the health benefits presented by the probiotic groups, the relative percentage survival at seven days following an intraperitoneal challenge with S. iniae (1010 CFU mL−1) was not different (11 %) between the groups and both presented 100 % infectivity. In summary, supplementing barramundi with B. subtilis via an oral or immersion route enhanced growth, upregulated intestinal gene expression, and changed the gut microbial community by increasing microbial diversity and adding potential beneficial microbes. Nevertheless, indirect ingestion of probiotics via an immersion may cause inconsistent effects; thus, oral supplementation is recommended to sustain an effective dosage of Bacillus probiotics in barramundi. © 2024 Elsevier B.V.

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