Protocol for culturing neurospheres from progenitor cells in the dentate gyrus of aged mouse hippocampus

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Publication Details

Author list: Vafaeva O.; Namchaiw P.; Murray K.; Diaz E.; Cheng H.-J.

Publisher: Cell Press

Publication year: 2025

Volume number: 6

Issue number: 1

ISSN:  2666-1667

eISSN: 2666-1667

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-86000603572&doi=10.1016%2fj.xpro.2025.103692&partnerID=40&md5=1816d2703e67a579da596db3a06483fd

Languages: English-Great Britain (EN-GB)

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Abstract

The neurosphere assay is the gold standard for assessing the proliferative and differentiation capacities of neural progenitor cells (NPCs). Here, we present a protocol for isolating, propagating, and maintaining hippocampal neurospheres from adult and aged mice and differentiating cultured NPCs into neurons and astrocytes. We describe steps for establishing a heterochronic co-culture of neurosphere-derived cells with primary neurons. Using neurospheres from old animals enables investigation of the effects of aging on the development and differentiation of newborn neurons. © 2025 The Author(s)

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