A comparative study of Thermomyces lanuginosus strains on thermostable xylanase production
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Publication Details
Author list: Khucharoenphaisan K., Tokuyama S., Ratanakhanokchai K., Kitpreechavanich V.
Publisher: Academic Journals
Publication year: 2009
Journal: African journal of Biotechnology (1684-5315)
Volume number: 8
Issue number: 8
Start page: 1608
End page: 1614
Number of pages: 7
ISSN: 1684-5315
eISSN: 1684-5315
Languages: English-Great Britain (EN-GB)
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Abstract
Strains of Thermomyces lanuginosus could be differentiated into two groups based on their ability to produce xylanase using xylan or xylose as sole of carbon source. One group of these strains produced high xylanase activity either in the medium using xylan or xylose as a sole of carbon source. The xylanase production by T. lanuginosus ATCC 44008, THKU-11, and THKU-25, which were the representative members of this group, increased when xylose was added to the xylan medium. In contrast, there was another group that produced high xylanase activity only in the xylan medium. Addition of xylose to the xylan medium resulted decreasing of xylanase production in T. lanuginosus ATCC 46882, TISTR 3465 and THKU-85 that belonged to this group. The finding indicated that induction and repression mechanism of xylose on xylanase expression was different among the strains of T. lanuginosus. In addition, phylogenetic analysis obtained from random amplified polymorphic DNA (RAPD) pattern using primer UBC 241 pointed to greater diversity of high and low xylanase producing strains using xylose as a carbon source. ฉ 2009 Academic Journals.
Keywords
RAPD, Thermomyces lanuginosus, Xylose induction and repression, β-xylanase
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