3D structure modeling of a transmembrane protein, fatty acid elongase

Conference proceedings article


ผู้เขียน/บรรณาธิการ


กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง


รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่งChumningan S., Pornputtapong N., Laoteng K., Cheevadhanarak S., Thammarongtham C.

ผู้เผยแพร่Springer Verlag (Germany): Computer Proceedings

ปีที่เผยแพร่ (ค.ศ.)2010

Volume number115 CCIS

หน้าแรก36

หน้าสุดท้าย45

จำนวนหน้า10

ISBN3642167497; 9783642167492

นอก1865-0929

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-78649516352&doi=10.1007%2f978-3-642-16750-8_4&partnerID=40&md5=772ad3bbfa7a7e891571f719ae744efa

ภาษาEnglish-Great Britain (EN-GB)


ดูในเว็บของวิทยาศาสตร์ | ดูบนเว็บไซต์ของสำนักพิมพ์ | บทความในเว็บของวิทยาศาสตร์


บทคัดย่อ

Fatty acid elongase is an enzyme responsible for fatty acid chain elongation, a key step in synthesis of long chain fatty acids, including polyunsaturated fatty acids (PUFAs). Currently, the increasing demand has raised the interest in obtaining these PUFAs from alternative sources, e.g. filamentous fungi that are more economical and sustainable. To date, many research on primary structures of fatty acid elongases ELO family, including fugal elongases, revealed several conserved motifs. However, molecular mechanism for their functions is still unclear. In addition to experimental study, computational analysis of elongase structures may provide more insight into their substrate specificities and mechanisms of fatty acid chain elongation. Thus, this work proposes a 3D structural model of elongase of Mortierella alpina (BAF97073). This fungal elongase has been reported to be a PUFA-specific elongation enzyme. The model was built by an ab initio membrane-modeling application using ROSETTA 3.1, and was then refined by molecular dynamic simulation. The 7-transmembrane helices of the constructed model folds into an anti-parallel configuration and embeds in the lipid bilayer. The model reveals that all four conserved signature motifs of fatty acid elongase enzymes are located within the juxta-cytosolic transmembrane helix regions. This work also suggests a modeling strategy of this elongase structural model that can be applied to model other transmembrane proteins. ฉ 2010 Springer-Verlag Berlin Heidelberg.


คำสำคัญ

ab initio ModelingFatty Acid ElongasePUFAsROSETTATransmembrane Protein


อัพเดทล่าสุด 2023-28-09 ถึง 07:35