Cross-linked enzyme crystals of organophosphate hydrolase for electrochemical detection of organophosphorus compounds
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Publication Details
Author list: Laothanachareon T., Champreda V., Sritongkham P., Somasundrum M., Surareungchai W.
Publisher: Springer
Publication year: 2008
Journal: World Journal of Microbiology and Biotechnology (0959-3993)
Volume number: 24
Issue number: 12
Start page: 3049
End page: 3055
Number of pages: 7
ISSN: 0959-3993
eISSN: 1573-0972
Languages: English-Great Britain (EN-GB)
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Abstract
Cross-linked enzyme crystals of organophosphate hydrolase (CLEC-OPH) prepared from crude recombinant E. coli cell lysate was used for the development of an electrochemical biosensor for the detection of organophosphate pesticides. CLEC-OPH showed an increased Vmax of 0.721 U mg protein-1 and a slightly lower Km of 0.083 mM on paraoxon compared to the crude enzyme, resulting in an improved catalytic efficiency (kcat/Km = 4.17 ื 105 M-1 min-1) with a remarkable increase on thermostability. An amperometric biosensor was constructed based on glutaraldehyde and albumin cross-linkage of CLEC-OPH with carbon nanotubes. The sensor exhibited greater sensitivity and operational stability with a lower limit of detection when compared with a sensor using an equivalent loading of crude OPH in a non-crystal form. The application of crude enzyme-based CLEC would offer a simple and economical approach for the fabrication of efficient electrochemical biosensors. ฉ 2008 The Author(s).
Keywords
Cross-linked enzyme crystal, Organophosphate hydrolase, Paraoxon
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