Sensitivity enhancement in DNA hybridization assay using gold nanoparticle-labeled two reporting probes
Journal article
Authors/Editors
Strategic Research Themes
No matching items found.
Publication Details
Author list: Oaew S., Karoonuthaisiri N., Surareungchai W.
Publisher: Elsevier
Publication year: 2009
Journal: Biosensors and Bioelectronics (0956-5663)
Volume number: 25
Issue number: 2
Start page: 435
End page: 441
Number of pages: 7
ISSN: 0956-5663
Languages: English-Great Britain (EN-GB)
View in Web of Science | View on publisher site | View citing articles in Web of Science
Abstract
A simple and sensitive method for DNA detection using gold nanoparticle (AuNP) two-probe detection system (AuNP-TP) was developed. Preliminary experiment was carried out by optimizing slide types, blocking agents and hybridization times. Fluorescent-labeled probes were used along with AuNP-labeled probes to confirm specific binding event between target DNA and probes. The sensitivities between AuNP single-probe (AuNP-SP) and AuNP-TP systems using sandwich-typed assay were compared. The AuNP-TP on epoxide-coated (EP) slides increased sensitivity 1000-fold at the detection limit of 100 fM when compared to the AuNP-SP. This result indicates that the assay sensitivity was simply enhanced by simultaneous adding two AuNP labeled probes which selectively recognize different regions of the target DNA. The concept of AuNP-TP could potentially be applied to a macroarray format to detect multiple DNA targets simultaneously; thereby making the assay becomes more affordable and more sensitive. ฉ 2009 Elsevier B.V. All rights reserved.
Keywords
DNA sensor, Fluorescent, Highly sensitive detection, Macroarray
Contact Information