A combined stress response analysis of Spirulina platensis in terms of global differentially expressed proteins, and mRNA levels and stability of fatty acid biosynthesis genes

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Jeamton W., Mungpakdee S., Sirijuntarut M., Prommeenate P., Cheevadhanarak S., Tanticharoen M., Hongsthong A.

ผู้เผยแพร่: Oxford University Press

ปีที่เผยแพร่ (ค.ศ.): 2008

วารสาร: FEMS Microbiology Letters (0378-1097)

Volume number: 281

Issue number: 2

หน้าแรก: 121

หน้าสุดท้าย: 131

จำนวนหน้า: 11

นอก: 0378-1097

eISSN: 1574-6968

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-41049107676&doi=10.1111%2fj.1574-6968.2008.01100.x&partnerID=40&md5=5440b4af48cc70fc2b033e92fcd1b714

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Changes in gene expression play a critical role in enhancing the ability of cyanobacteria to survive under cold conditions. In the present study, Spirulina platensis cultures were grown at the optimal growth temperature, in the light, before being transferred to dark conditions at 22 °C. Two dimensional-differential gel electrophoresis was then performed to separate differentially expressed proteins that were subsequently identified by MS. Among all differentiated proteins identified, a protein involved in fatty acid biosynthesis, (3R)-hydroxymyristoyl-[acyl-carrier-protein]-dehydratase encoded by fabZ, was the most up-regulated protein. However, the fatty-acid desaturation proteins were not significantly differentiated. This raised the question of how the unsaturated fatty acid, especially γ-linolenic acid, content in the cells in the cold-dark shift remained stable compared with that of the cold shift. Thus, a study at the transcriptional level of these desaturase genes, desC, desA and desD, and also of the fabZ gene was conducted. The results indicated that in the dark, where energy is limited, mRNA stability was enhanced by exposure to low temperatures. The data demonstrate that when the cells encounter cold stress with energy limitation, they can maintain their homeoviscous adaptation ability via mRNA stability. © 2008 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd.

คำสำคัญ

2D-DIGE, Cold shock, Fatty acid biosynthesis genes, Light-to-dark shift, mRNA stability