Proteome analysis at the subcellular level of the cyanobacterium Spirulina platensis in response to low-temperature stress conditions

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Hongsthong A., Sirijuntarut M., Prommeenate P., Lertladaluck K., Porkaew K., Cheevadhanarak S., Tanticharoen M.

ผู้เผยแพร่: Oxford University Press

ปีที่เผยแพร่ (ค.ศ.): 2008

วารสาร: FEMS Microbiology Letters (0378-1097)

Volume number: 288

Issue number: 1

หน้าแรก: 92

หน้าสุดท้าย: 101

จำนวนหน้า: 10

นอก: 0378-1097

eISSN: 1574-6968

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-52649150900&doi=10.1111%2fj.1574-6968.2008.01330.x&partnerID=40&md5=b281434feed3ba6c5f95680a56ae7408

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

The present study addresses the differential expression of Spirulina platensis proteins detected during cold-induced stress, analyzed at the subcellular level. In performing differential expression analysis, the results revealed upregulated proteins in every subcellular fraction, including two-component response systems, DNA repair, molecular chaperones, stress-induced proteins and proteins involved in other biological processes such as secretion systems and nitrogen assimilation. The chlorophyll biosynthetic proteins, protochlorophyllide oxidoreductase and ChlI, had unique expression patterns as detected in the thylakoid membrane; the levels of these proteins immediately decreased during the first 45 min of low-temperature exposure. In contrast, their expression levels significantly increased after low-temperature exposure, indicating the relevance of the chlorophyll biosynthesis in Spirulina in response to low-temperature stress in the light condition. In addition, this is the first report in which genome-based protein identification in S. platensis by peptide mass fingerprinting was performed using the database derived from the unpublished Spirulina genome sequence. ฉ 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

คำสำคัญ

Low-temperature stress, Proteome analysis, S. platensis and differential expression