Effect of carbon sources on the induction of xylanolytic-cellulolytic multienzyme complexes in Paenibacillus curdlanolyticus strain B-6

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Publication Details

Author list: Waeonukul R., Kyu K.L., Sakka K., Ratanakhanokchai K.

Publisher: Taylor & Francis: STM, Behavioural Science and Public Health Titles

Publication year: 2008

Journal: Bioscience, Biotechnology and Biochemistry (0916-8451)

Volume number: 72

Issue number: 2

Start page: 321

End page: 328

Number of pages: 8

ISSN: 0916-8451

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-40449132768&doi=10.1271%2fbbb.70333&partnerID=40&md5=ec723a5ef57eb25a5122602d2490445e

Languages: English-Great Britain (EN-GB)

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Abstract

The effect of polymeric substances such as α-cellulose, birchwood xylan, corn hull, and sugarcane bagasse, and of soluble sugars such as L-arabinose, D-galactose, D-glucose, D-xylose, and cellobiose, on the induction of multienzyme complexes in a facultatively anaerobic bacterium, Paenibacillus curdlanolyticus B-6, was investigated under aerobic conditions. Cells and culture supernatants of strain B-6 grown on different carbon sources were analyzed. Cells grown on each carbon source adhered to cellulose. Hence strain B-6 cells from all carbon sources must have an essential component responsible for anchoring the cells to the substrate surfaces. Native-polyacrylamide gel electrophoresis (native-PAGE), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), zymogram analysis, and enzymatic assays indicated that many proteins having xylanolytic and cellulolytic activities from P. curdlanolyticus B-6 grown on each carbon source were produced as two multienzyme complexes in the culture supernatants. These results indicate that P. curdlanolyticus B-6 produced multienzyme complexes when grown on both polymeric and soluble sugars. The multienzyme complexes of P. curdlanolyticus B-6 consisted of the main enzymes and non-enzymatic subunits and the production of some different subunits, depending on the carbon source.

Keywords

Carbon sources, Cellulose-binding