Analysis of ORF27 full length sequence of Thai isolated Helicoverpa armigera nucleopolyhedrovirus and prokaryotic expression of truncated ORF27 gene

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Xun M., Poomputsa K.

ปีที่เผยแพร่ (ค.ศ.): 2010

วารสาร: Journal of Xi'an Jiaotong University (Medical Sciences) (1671-8259)

Volume number: 31

Issue number: 3

หน้าแรก: 293

หน้าสุดท้าย: 298

จำนวนหน้า: 6

นอก: 1671-8259

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-77952737340&partnerID=40&md5=0e6420e27b6a77c92dcc5ef1bfb58ed2

บทคัดย่อ

Objective: To understand the functions of ORF27 protein of the Thai isolated Helicoverpa armigera nucleopolyhedrovirus (HaNPV-Th) and explain the possible molecular mechanism of HaNPV-Th in passage effect. Methods: ORF27 gene was cloned and amplified by polymerase chain reaction (PCR) from HaNPV-Th genome. The physical-chemical properties, subcellular localization, signal peptide, transmembrane structure and functional motifs were analyzed by the software of ProtParam, TMpred, SignalP and ScanProsite. The truncated ORF27 gene which lacked parts of the amino-terminal was expressed by bacterial expression system. Results: The full length sequence of ORF27 was 768bp long and encoded 255aa with a molecular weight of 29.5ku. Several functional motifs on ORF27 were predicted by bioinformatics software. The truncated ORF27 recombinant expressed 35ku target protein mainly in the form of inclusion body. Conclusion: ORF27 protein of HaNPV-Th is presumed to possess extensive biological activities. It may regulate various cellular signaling pathways, apoptosis and cell cycle, which are involved in passage effect.

คำสำคัญ

ORF27, Passage effect, Prokaryotic expression, Sequence analysis, Thai isolated Helicoverpa armigera nucleopolyhedrovirus (HaNPV-Th)