Identifying parameters influencing growth and astaxanthin production by Xanthophyllomyces dendrorhous cultivated in pineapple juice concentrate base low cost medium
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Publication Details
Author list: Abdullah S., Poomputsa K., Mekvichitsaeng P., Ruanglek V., Akeprathumchai S.
Publication year: 2011
Journal: Thai Journal of Agricultural Science (0049-3589)
Volume number: 44
Issue number: 5
Start page: 89
End page: 94
Number of pages: 6
ISSN: 0049-3589
Languages: English-Great Britain (EN-GB)
Abstract
Astaxanthin, a potent antioxidant, has gained considerable attention in several applications such as food, feed, and pharmaceutical industries. However, synthetic astaxanthin is expensive and poses safety concern when applying as feed additive. Therefore, biologically synthesized astaxanthin represents a safer alternative. Xanthophyllomyces dendrorhous is the red yeast capable of synthesizing astaxanthin as its major carotenoid pigment. As wild types in general produce low pigment concentration, objective of this study was to identify parameters significantly influencing growth as well as astaxanthin production by X. dendrorhous cultivated on pineapple juice concentrate based growth medium, a sugar as well as amino acids rich agricultural waste derived from canned pineapple manufacturing process. Screening conducted on six culturing factors, i.e., sucrose, glucose, (NH4)2SO4, KNO3, nhexadecane, and pH was accomplished using fractional factorial design (FFD). Results showed that sucrose, KNO3, and n-hexadecane were identified as the significant factors affecting cell growth (p<0.05) while sucrose, glucose, (NH4)2SO4, KNO3, and n-hexadecane were considered statistically significant for astaxanthin production. Results further suggested that increasing cell growth would increase astaxanthin production since astaxanthin production is directly proportional to the concentration of biomass. Therefore, the most important factors, namely, sucrose, KNO3, and n-hexadecane, were adopted for further optimization study by Doehlert design.
Keywords
FFD, Pineapple juice concentrates, Screening, X. dendrorhous
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