A colorimetric assay for determination of methyl parathion using recombinant methyl parathion hydrolase
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Author list: Anh D.H., Cheunrungsikul K., Wichitwechkarn J., Surareungchai W.
Publication year: 2011
Volume number: 6
Issue number: 5
Start page: 565
End page: 571
Number of pages: 7
ISSN: 1860-6768
eISSN: 1860-6768
Languages: English-Great Britain (EN-GB)
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Abstract
A simple, rapid and sensitive colorimetric dipstick assay for the detection of the organophosphorous insecticide methyl parathion (MPT) residue in vegetables was developed. The assay was based on the hydrolysis of MPT by a recombinant methyl parathion hydrolase (recMPH), the encoding gene of which was isolated from Burkholderia cepacia, a soil bacterium indigenous to Thailand. This reaction generates protons leading to a change in pH that correlates with the amount of MPH present. Hence, the pH indicator bromothymol blue was used to monitor the MPH hydrolysis as the associated color changes can be observed by the naked eye. The recMPH was immobilized on a PVDF membrane to establish a dipstick assay format. The assays could detect MPT residues in spiked vegetable samples at the concentration of 1 mg/L without using analytical instrumentation. The test is reusable and stable for up to 3 months in the absence of any preservatives. ฉ 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Keywords
Bromothymol blue, Colorimetric assay, Methyl parathion, Recombinant methyl parathion hydrolase
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