Oxygen-induced expression of Δ6-, Δ9- and Δ12-desaturase genes modulates fatty acid composition in Mucor rouxii
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Author list: Ruenwai R., Cheevadhanarak S., Rachdawong S., Tanticharoen M., Laoteng K.
Publisher: Springer
Publication year: 2010
Journal: Applied Microbiology and Biotechnology (0175-7598)
Volume number: 86
Issue number: 1
Start page: 327
End page: 334
Number of pages: 8
ISSN: 0175-7598
eISSN: 1432-0614
Languages: English-Great Britain (EN-GB)
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Abstract
The effect of oxygen availability on the molecular mechanisms of fatty acid biosynthesis was investigated in Mucor rouxii, a Mucorale fungus capable of producing γ-linolenic acid through perturbation of the gaseous environment. Shifting of the M. rouxii culture from anaerobic to aerobic conditions resulted in an increase of the biomass and total fatty acid content of the M. rouxii culture. In addition, the levels of unsaturated fatty acids were enhanced accompanied by a decrease in the levels of medium- and long-chain saturated fatty acids. These results correspond to the levels of expressions of the Δ9-, Δ12- and Δ6- desaturases genes, all of which were coordinately up-regulated after the shift. The transcriptional response observed was rapid and transient, with the maximal mRNA levels detected between 0.5 h and 1.0 h after the shift. Together, our findings indicate that the anaerobic M. rouxii culture acclimatised to oxygen exposure by modulating fatty acid composition that was transcriptionally co-regulated by the Δ9-, Δ12- and Δ6-desaturase genes. © 2009 Springer-Verlag.
Keywords
Gamma-linolenic acid, Gene expression, Mucor rouxii, Oxygen
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