Improving the remaining activity of lignocellulolytic enzymes by membrane entrapment
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Author list: Chang K.-L., Thitikorn-amorn J., Chen S.-H., Hsieh J.-F., Ratanakhanokchai K., Huang P.-J., Lin T.-C., Chen S.-T.
Publisher: Elsevier
Publication year: 2011
Journal: Bioresource Technology (0960-8524)
Volume number: 102
Issue number: 2
Start page: 519
End page: 523
Number of pages: 5
ISSN: 0960-8524
Languages: English-Great Britain (EN-GB)
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Abstract
The production of bioethanol by the conversion of lignocellulosic waste has attracted much interest in recent years because of its low cost and great potential availability. However, the high cost of the enzyme required for this conversion is often considered to be the major bottleneck in the commercial lignocellulosic ethanol industry. In this work, the hydrolysis of rice straw by free and entrapped lignocellulolytic enzymes (cellulase, xylanase and laccase) was carried out at pH 5.5 and 37 ฐC. The hydrolysis of rice straw by enzymes entrapped in a membrane produced a higher monosaccharide content: 601.05. mg/g rice straw for entrapped enzymes vs. 465.46. mg/g rice straw for free enzymes. This study has shown that enzyme entrapment is an important technique for the efficient use and reuse of enzymes in industrial applications and also for the rapid separation of saccharide products from the reaction medium, thus improving the remaining enzymatic activities. ฉ 2010 Elsevier Ltd.
Keywords
Entrapment, Enzyme activity, Inhibitor
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