Immunoassay based on carbon nanotubes-enhanced ELISA for Salmonella enterica serovar Typhimurium
Journal article
Authors/Editors
Strategic Research Themes
No matching items found.
Publication Details
Author list: Chunglok W., Wuragil D.K., Oaew S., Somasundrum M., Surareungchai W.
Publisher: Elsevier
Publication year: 2011
Journal: Biosensors and Bioelectronics (0956-5663)
Volume number: 26
Issue number: 8
Start page: 3584
End page: 3589
Number of pages: 6
ISSN: 0956-5663
Languages: English-Great Britain (EN-GB)
View in Web of Science | View on publisher site | View citing articles in Web of Science
Abstract
Among the methods used to detect pathogenic bacteria, enzyme linked immunosorbent assay (ELISA) is one of the most widely used techniques in routine sample analysis. For Salmonella enterica serovar Typhimurium detection, a typical ELISA yields a sensitivity of 106-107CFU/ml. To enhance the detection sensitivity, single-walled carbon nanotubes (SWCNTs) was employed in this study as a labelling platform for antibody and horseradish peroxidase (HRP) co-immobilizing. With high proteins recovery after the coupling process, the resulting Ab/SWCNTs/HRP bioconjugate was used in the proof-of-concept ELISA experiments. Limit of detection (LOD) was found to be 103 and 104CFU/ml for direct and sandwich ELISA, respectively, when Ab/HRP at 1:400 ratio was used. This figure accounts for 1000-time greater in detection sensitivity when compared to a commercial Ab-HRP conjugate. The Ab/SWCNTs/HRP bioconjugate was tested further in real samples and found a superior activity over the commercial Ab-HRP by showing 100-time greater detection limit. ฉ 2011 Elsevier B.V.
Keywords
ELISA, Salmonella enterica serovar Typhimurium
Contact Information