Identification of a heat shock-responsive cis-acting DNA sequence and its transcriptional regulator: Their roles in the expression of the Spirulina-desD gene in response to heat stress

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Author list: Kurdrid P., Phuengcharoen P., Cheevadhanarak S., Tanticharoen M., Hongsthong A.

Publisher: Elsevier

Publication year: 2010

Journal: Journal of Bioscience and Bioengineering (1389-1723)

Volume number: 109

Issue number: 3

Start page: 205

End page: 210

Number of pages: 6

ISSN: 1389-1723

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-76249112315&doi=10.1016%2fj.jbiosc.2009.09.002&partnerID=40&md5=d8c95dcd7d408da80ec5ded93eb8ac8d

Languages: English-Great Britain (EN-GB)

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Abstract

This study addresses the importance of a heat-shock-responsive cis-acting DNA element and its transcriptional regulator, which play key roles in the regulation of the Spirulina-desD gene on exposure to high temperatures. Temperature response analysis studies showed that the AT-rich region that is located between nt - 98 to - 80 of the Spirulina-desD gene promoter serves as a binding site for its transcriptional regulator. LC-MS/MS analysis of the DNA-binding protein complex revealed that the amino acid sequences of the bound proteins were homologous to those of several proteins, including a DNA-binding protein, heat shock protein-90 (Hsp90 or HtpG), GroEL and various protein kinases. In addition, western blot analysis indicated that the chaperones GroEL and Hsp90 and a dephosphorylation reaction played a role in the response to elevated temperatures. We conclude that the regulatory DNA segments and the corresponding regulatory binding proteins are distinct for each particular stress condition. This is true, irrespective of whether the regulatory mechanisms that govern the expression of the cold- and heat-regulated desD gene depend on similar phosphorylation- and dephosphorylation-dependent conformational changes that modulate the association of the co-chaperone. ฉ 2009 The Society for Biotechnology, Japan.

Keywords

AT-rich region, Regulatory protein