Biocatalytic ethanolysis of palm oil for biodiesel production using microcrystalline lipase in tert-butanol system

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Raita M., Champreda V., Laosiripojana N.

ผู้เผยแพร่: Elsevier

ปีที่เผยแพร่ (ค.ศ.): 2010

วารสาร: Process Biochemistry (1359-5113)

Volume number: 45

Issue number: 6

หน้าแรก: 829

หน้าสุดท้าย: 834

จำนวนหน้า: 6

นอก: 1359-5113

eISSN: 1873-3298

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-77951024962&doi=10.1016%2fj.procbio.2010.02.002&partnerID=40&md5=1b12299584112792a95a312a29744793

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Biocatalytic synthesis is a promising environmentally friendly process for the production of biodiesel, a sustainable alternative fuel from renewable plant resources. In order to develop an economical heterogeneous biocatalyst, protein-coated microcrystals (PCMCs) were prepared from a commercial enzyme preparation from a recombinant Aspergillus strain expressing Thermomyces lanuginosus lipase and used for synthesis of biodiesel from palm olein by ethanolysis. Reaction parameters, including catalyst loading, temperature, and oil/alcohol molar ratio have been systematically optimized. Addition of tert-butanol was found to markedly increase the biocatalyst activity and stability resulting in improved product yield. Optimized reactions (20%, w/w PCMC-lipase to triacylglycerol and 1:4 fatty acid equivalence/ethanol molar ratio) led to the production of alkyl esters from palm olein at 89.9% yield on molar basis after incubation at 45 ฐC for 24 h in the presence of tert-butanol at a 1:1 molar ratio to triacylglycerol. Crude palm oil and palm fatty acid distillate were also efficiently converted to biodiesel with 82.1 and 75.5% yield, respectively, with continual dehydration by molecular sieving. Operational stability of PCMC-lipase could be improved by treatment with tert-butanol allowing recycling of the biocatalyst for at least 8 consecutive batches with only slight reduction in activity. This work thus shows a promising approach for biodiesel synthesis with microcrystalline lipase which could be further developed for cost-efficient industrial production of biodiesel. ฉ 2010 Elsevier Ltd. All rights reserved.

คำสำคัญ

Ethanolysis, Lipase, Protein-coated microcrystals