Electrochemical immunoassay platform for high sensitivity protein detection based on redox-modified carbon nanotube labels
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Author list: Chunglok W., Khownarumit P., Rijiravanich P., Somasundrum M., Surareungchai W.
Publisher: Royal Society of Chemistry
Publication year: 2011
Journal: Analyst (0003-2654)
Volume number: 136
Issue number: 14
Start page: 2969
End page: 2974
Number of pages: 6
ISSN: 0003-2654
eISSN: 1364-5528
Languages: English-Great Britain (EN-GB)
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Abstract
We report a highly sensitive immunoassay protocol based on the use of redox-modified multi-walled carbon nanotubes (MWNTs) as electrochemical labels. The MWNTs were coated with methylene blue (MB) at an optically-determined loading of 3.41 ื 10-3 mol g-1, and were then attached to secondary antibodies (Ab2) by adsorption. As a model analyte mouse IgG was collected by primary antibody (Ab1)-coated magnetic beads. Following binding of the MB-MWNT-Ab2 conjugates, IgG could be measured by MB reduction. Using differential pulse voltammetry for quantification, IgG was calibrated with a dynamic range of 0.1 pg mL -1 to 100 pg mL-1. Given the different possible Ab 1-MB-MWNT-Ab2 orientations on the magnetic beads, it was likely that not all the MB communicated with the electrode. A greater quantity of MB could be accessed by using the Fe(CN)63-/4- redox couple as a solution phase mediator. This enabled us to lower the dynamic range down to 5 fg mL-1 to 100 fg mL-1. ฉ The Royal Society of Chemistry 2011.
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