Comparative analysis of the Spirulina platensis subcellular proteome in response to low- and high-temperature stresses: Uncovering cross-talk of signaling components

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Kurdrid P., Senachak J., Sirijuntarut M., Yutthanasirikul R., Phuengcharoen P., Jeamton W., Roytrakul S., Cheevadhanarak S., Hongsthong A.

ปีที่เผยแพร่ (ค.ศ.): 2011

Volume number: 9

นอก: 1477-5956

eISSN: 1477-5956

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960555061&doi=10.1186%2f1477-5956-9-39&partnerID=40&md5=39a8fa86955944705fb39359e58f837a

ภาษา: English-Great Britain (EN-GB)

ดูในเว็บของวิทยาศาสตร์ | ดูบนเว็บไซต์ของสำนักพิมพ์ | บทความในเว็บของวิทยาศาสตร์

บทคัดย่อ

The present study focused on comparative proteome analyses of low- and high-temperature stresses and potential protein-protein interaction networks, constructed by using a bioinformatics approach, in response to both stress conditions. The data revealed two important points: first, the results indicate that low-temperature stress is tightly linked with oxidative stress as well as photosynthesis; however, no specific mechanism is revealed in the case of the high-temperature stress response. Second, temperature stress was revealed to be linked with nitrogen and ammonia assimilation. Moreover, the data also highlighted the cross-talk of signaling pathways. Some of the detected signaling proteins, e.g., Hik14, Hik26 and Hik28, have potential interactions with differentially expressed proteins identified in both temperature stress conditions. Some differentially expressed proteins found in the Spirulina protein-protein interaction network were also examined for their physical interactions by a yeast two hybrid system (Y2H). The Y2H results obtained in this study suggests that the potential PPI network gives quite reliable potential interactions for Spirulina. Therefore, the bioinformatics approach employed in this study helps in the analysis of phenomena where proteome analyses of knockout mutants have not been carried out to directly examine for specificity or cross-talk of signaling components. ฉ 2011 Kurdrid et al; licensee BioMed Central Ltd.

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