Characterization of Paenibacillus curdlanolyticus intracellular xylanase xyn10B encoded by the xyn10B gene

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Publication Details

Author list: Sudo M., Sakka M., Kimura T., Ratanakhanokchai K., Sakka K.

Publisher: Taylor & Francis: STM, Behavioural Science and Public Health Titles

Publication year: 2010

Journal: Bioscience, Biotechnology and Biochemistry (0916-8451)

Volume number: 74

Issue number: 11

Start page: 2358

End page: 2360

Number of pages: 3

ISSN: 0916-8451

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-78649771410&doi=10.1271%2fbbb.100555&partnerID=40&md5=8b27d5eef529e207b6643f85d53e3cf2

Languages: English-Great Britain (EN-GB)

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Abstract

The Paenibacillus curdlanolyticus xyn10B gene encoding a family-10 xylanase was cloned and expressed in Escherichia coli, and the recombinant enzyme rXyn10B was characterized. Immunological analysis suggested that Xyn10B is an intracellular enzyme. rXyn10B hydrolyzed birch-wood xylan and xylooligosaccharides to produce mainly xylobiose, suggesting that it is an endoxylanase. Its properties were significantly different from those of some homologous enzymes.

Keywords

Paenibacillus curdlanolyticus