Involvement of chlorophyllase on chlorophyll degradation in stored broccoli florets and its control by UV treatment
Conference proceedings article
ผู้เขียน/บรรณาธิการ
กลุ่มสาขาการวิจัยเชิงกลยุทธ์
ไม่พบข้อมูลที่เกี่ยวข้อง
รายละเอียดสำหรับงานพิมพ์
รายชื่อผู้แต่ง: Kaosamphan A., Yamauchi N., Srilaong V., Aiamla-Or S., Wongs-Aree C., Uthairatanakij A.
ผู้เผยแพร่: International Society for Horticultural Science (ISHS)
ปีที่เผยแพร่ (ค.ศ.): 2010
Volume number: 875
หน้าแรก: 111
หน้าสุดท้าย: 118
จำนวนหน้า: 8
ISBN: 9789066055636
นอก: 0567-7572
eISSN: 2406-6168
ภาษา: English-Great Britain (EN-GB)
บทคัดย่อ
Yellowing is the most visible deterioration in broccoli (Brassica oleracea L. Italica Group) that usually occurs with the progress of chlorophyll (Chl) degradation. This study deals with the control of floret yellowing in stored broccoli by using UV-B irradiation at 0 (control) and 8.8 kJ.m-2. The application of UV-B at 8.8 kJ.m-2 effectively delayed yellowing of broccoli florets. Chl a derivatives including chlorophyllide (Chlide) a, C132-hydroxychlorophyll (C132-OHChl) a and pheophytin (Phy) a levels in broccoli florets were decreased concomitantly with the enhancement of pheophorbide (Pheide) a and pyropheophorbide (Pyropheide) a levels especially in the control treatment. On the other hand, Chlide a, C132-OHChl a and Phy a levels were increased in 8.8 kJ.m-2 UV-B treated broccoli during storage at 15ฐC. Two types of chlorophyllase (Chlase) were identified by molecular exclusion chromatography. Type I was suppressed in UV-B treated broccoli on day 4, and the Km value of Type I was lower than that of Type II. Thus, the Chlase activity, especially Type I, was effectively suppressed by UV-B treatment, whereas Type II Chlase may take in part of Chl degradation in stored broccoli florets.
คำสำคัญ
Broccoli, Chlorophyllase, Chlorophyll derivatives
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