Up-regulated expression of desaturase genes of Mucor rouxii in response to low temperature associates with pre-existing cellular fatty acid constituents
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Author list: Cheawchanlertfa P., Cheevadhanarak S., Tanticharoen M., Maresca B., Laoteng K.
Publisher: Springer
Publication year: 2011
Journal: Molecular Biology Reports (0301-4851)
Volume number: 38
Issue number: 5
Start page: 3455
End page: 3462
Number of pages: 8
ISSN: 0301-4851
eISSN: 1573-4978
Languages: English-Great Britain (EN-GB)
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Abstract
Transcriptional response of desaturase genes to low temperature was investigated in the dimorphic fungus Mucor rouxii. The two morphological forms of M. rouxii, yeast-like and mycelial cells containing different fatty acid profiles were shifted from 30 to 10°C. Both cultures exhibited significantly altered fatty acid composition, whose content in polyunsaturated fatty acids increased as consequence of the temperature shift and was accompanied by a reduction of C18:1Δ9 about 2 h after the temperature shift. These changes were particularly significant in phosphatidylcholine and phosphatidylethanolamine fractions. Moreover, the fatty acid profiles of monoacylglycerol and diacylglycerol were also modulated in response to the lower temperature of incubation. The changes of membrane lipids of M. rouxii were due to the cold-induced expression of Δ9-, Δ12- and Δ6-desaturase genes. Although the mRNA levels of the three desaturases were transiently induced by lowering the temperature, the pre-existing composition of fatty acid profiles of mycelial and yeast-like forms of M. rouxii may have lead to different expression profiles of desaturase genes that modified their membrane physical state under cold shock. While expression of Δ12-desaturase gene contributedmainly to cold acclimation of mycelia, Δ9-desaturase expression was the main transcript identified in the yeast-like culture after temperature shift. © Springer Science+Business Media B.V. 2010.
Keywords
Cell morphology, Low temperature
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