Production and accumulation of 4-o-methyl-α-D-glucuronosyl-xylotriose by growing culture of thermophilic Anoxybacillus sp. strain JT-12
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Author list: Thitikorn-Amorn J., Kyu K.L., Sakka K., Ratanakhanokchai K.
Publisher: Springer
Publication year: 2012
Journal: Applied Biochemistry and Biotechnology (0273-2289)
Volume number: 166
Issue number: 7
Start page: 1791
End page: 1800
Number of pages: 10
ISSN: 0273-2289
eISSN: 1559-0291
Languages: English-Great Britain (EN-GB)
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Abstract
A thermophilic Anoxybacillus sp. strain JT-12, isolated from soil, produced acidic xylotriose, 4-O-methyl-a-D-glucuronosyl-xylotriose (MeGlcAX 3), as a main product from birchwood xylan and accumulated them in the culture under optimum conditions at pH 7.0 and 55 ฐC using 0.75% (w/v) birchwood xylan as a carbon source for 42-72 h. The acidic xylotriose was purified by ethanol precipitation and high-performance liquid chromatography using NH2 Lichosherฎ 100 column. The results of electrospray ionization mass spectrometry, mass to charge ratio (m/z) 603.23, confirmed that the purified sample was acidic xylotriose that had benefits and applications in many fields. ฉ Springer Science+Business Media, LLC 2012.
Keywords
4-O-methyl-a-D-glucuronosylxylotriose
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