Queen production and instrumental insemination of Apis florea queens
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Author list: Phiancharoen M., Wongsiri S., Hepburn H.R.
Publisher: Springer
Publication year: 2011
Journal: Apidologie (0044-8435)
Volume number: 42
Issue number: 3
Start page: 307
End page: 311
Number of pages: 5
ISSN: 0044-8435
eISSN: 1297-9678
Languages: English-Great Britain (EN-GB)
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Abstract
Virgin queens of A. florea were produced in 10 queenless colonies yielding 106 queens with an average of 10.6ฑ2.99 queen cells per colony and a success rate of 65.23ฑ0.14% virgin queens. Spermatozoa were collected directly from the seminal vesicles. Thirty queens were inseminated, each with a pool of about 3.12ื10 6 spermatozoa derived from 8 drones. Six queens began to lay eggs 5 to 14 days after instrumental insemination. The mean number of spermatozoa reaching the spermatheca of inseminated queens was 0.74ื 10 6ฑ0.45 (=24% of the drone's spermatozoa) and the percentage of worker offspring was 100% in 5 queens and 83% in one queen. This method opens the possibility for new studies in genetics and selective breeding. ฉ INRA, DIB-AGIB and Springer Science+Business Media B.V., 2011.
Keywords
Instrumental insemination, Offspring, Queen production, Spermatozoa
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