Efficient saccharification of ammonia soaked rice straw by combination of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii β-glucosidase
Journal article
Authors/Editors
Strategic Research Themes
No matching items found.
Publication Details
Author list: Waeonukul R., Kosugi A., Tachaapaikoon C., Pason P., Ratanakhanokchai K., Prawitwong P., Deng L., Saito M., Mori Y.
Publisher: Elsevier
Publication year: 2012
Journal: Bioresource Technology (0960-8524)
Volume number: 107
Start page: 352
End page: 357
Number of pages: 6
ISSN: 0960-8524
Languages: English-Great Britain (EN-GB)
View in Web of Science | View on publisher site | View citing articles in Web of Science
Abstract
Clostridium thermocellum is known to produce the cellulosomes with efficient plant cell wall degradation ability. To bring out the maximum cellulolytic ability of the cellulosomes, it is necessary to eliminate the end product inhibition by cellobiose. Combinations of β-glucosidases from thermophilic anaerobic bacteria and Aspergillus niger and C. thermocellum S14 cellulosomes were evaluated for optimization of cellulose degradation β-Glucosidase (CglT) from Thermoanaerobacter brockii, in combination with cellulosomes, exhibited remarkable saccharification ability for microcrystalline cellulose. When rice straw, soaked in 28% aqueous ammonia for 7. days at 60 °C, was hydrolyzed by an enzyme loading combination of 2. mg cellulosome and 10 units CglT per g glucan, 91% of glucan was hydrolyzed to glucose, indicating roughly1/10 the enzyme load of a Trichoderma reesei cellulase (Celluclast 1.5L) and Novozyme-188 combination is enough for the combination of C. thermocellum S14 cellulosomes and CglT to achieve the same level of saccharification of rice straw. © 2011 Elsevier Ltd.
Keywords
Cellulosome, Clostridium thermocellum, Enzyme loading, Thermoanaerobacter brockii, β-Glucosidase
Contact Information