Optimisation of synergistic biomass-degrading enzyme systems for efficient rice straw hydrolysis using an experimental mixture design

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Author list: Suwannarangsee S., Bunterngsook B., Arnthong J., Paemanee A., Thamchaipenet A., Eurwilaichitr L., Laosiripojana N., Champreda V.

Publisher: Elsevier

Publication year: 2012

Journal: Bioresource Technology (0960-8524)

Volume number: 119

Start page: 252

End page: 261

Number of pages: 10

ISSN: 0960-8524

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862673640&doi=10.1016%2fj.biortech.2012.05.098&partnerID=40&md5=0c395a405d0ca54d4091a50a698c79a5

Languages: English-Great Britain (EN-GB)

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Abstract

Synergistic enzyme system for the hydrolysis of alkali-pretreated rice straw was optimised based on the synergy of crude fungal enzyme extracts with a commercial cellulase (Celluclast™). Among 13 enzyme extracts, the enzyme preparation from Aspergillus aculeatus BCC 199 exhibited the highest level of synergy with Celluclast™. This synergy was based on the complementary cellulolytic and hemicellulolytic activities of the BCC 199 enzyme extract. A mixture design was used to optimise the ternary enzyme complex based on the synergistic enzyme mixture with Bacillus subtilis expansin. Using the full cubic model, the optimal formulation of the enzyme mixture was predicted to the percentage of Celluclast™: BCC 199: expansin = 41.4:37.0:21.6, which produced 769. mg reducing sugar/g. biomass using 2.82. FPU/g enzymes. This work demonstrated the use of a systematic approach for the design and optimisation of a synergistic enzyme mixture of fungal enzymes and expansin for lignocellulosic degradation. © 2012 Elsevier Ltd.

Keywords

Lignocellulose hydrolysis, Mixture design