RGB colour coding of Y-shaped DNA for simultaneous tri-analyte solid phase hybridization detection
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Publication Details
Author list: Krissanaprasit A., Somasundrum M., Surareungchai W.
Publisher: Elsevier
Publication year: 2011
Journal: Biosensors and Bioelectronics (0956-5663)
Volume number: 26
Issue number: 5
Start page: 2183
End page: 2187
Number of pages: 5
ISSN: 0956-5663
Languages: English-Great Britain (EN-GB)
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Abstract
We present a new concept for tri-analyte DNA detection based on the idea of a Y-shaped capture probe which, after tri-target and fluorescently labeled reporter probe binding, becomes colour-coded to generate images in an RGB colour scheme. Hence, the RGB value of the resulting secondary pseudo-colour presented by the hybridized Y-DNA can be related to the ratio of the primary pseudo-colours present in its make-up, and thus to the ratio of the three target concentrations. As a proof of concept we detect sequences from the genes of the pathogenic bacterial strains Escherichia coli O157:H7, Vibrio cholera and Salmonella enteric in a semi-quantitative manner across the range 20-167. nM. The assay was relatively quick, with a time from hybridization to completed data interpretation of approximately 4. h. ฉ 2010 Elsevier B.V.
Keywords
Colocalization, DNA detection, Multicolour, Multiplex
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