Development of a one-step immunochromatographic strip test using gold nanoparticles for the rapid detection of Salmonella typhi in human serum

Journal article

Authors/Editors

WIJITAR DUNGCHAI

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Publication Details

Author list: Preechakasedkit P., Pinwattana K., Dungchai W., Siangproh W., Chaicumpa W., Tongtawe P., Chailapakul O.

Publisher: Elsevier

Publication year: 2012

Journal: Biosensors and Bioelectronics (0956-5663)

Volume number: 31

Issue number: 1

Start page: 562

End page: 566

Number of pages: 5

ISSN: 0956-5663

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84455208100&doi=10.1016%2fj.bios.2011.10.031&partnerID=40&md5=84938ebbe31e1075304c2f4a1b4a6841

Languages: English-Great Britain (EN-GB)

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Abstract

An immunochromatographic strip test using gold nanoparticles was developed for the rapid detection of Salmonella typhi (S. typhi) in human serum. The strip test based on the principle of sandwich immunoassay by the specific binding of antigens from S. typhi O901 and antibody of S. typhi O901 on a nitrocellulose membrane. Antibody-gold nanoparticle conjugate was used as the label and was coated onto a glass fiber membrane, which was used as a conjugate pad. To create a test and control zone, antibody of S. typhi O901 and an anti-IgG were dotted on the nitrocellulose membrane, respectively. Positive samples were displayed as red dots at the test and control zones of the nitrocellulose membrane, while negative samples resulted in a red dot only in the control zone. The limit of detection (LOD) was found to be 1.14ื10 5cfumL -1, which could be visually detected by the naked eye within 15min. This strip test provided a lower detection limit and analysis time than a dot blot immunoassay (8.88ื10 6cfumL -1 for LOD and 110min for reaction time). In addition, our immunochromatographic strip test was employed to detect S. typhi in human serum effectively, with high accuracy. This strip test offers great promise for a rapid, simple and low-cost analysis of S. typhi. ฉ 2011 Elsevier B.V.

Keywords

Immunochromatographic strip test