Gold nanoparticles/horseradish peroxidase encapsulated polyelectrolyte nanocapsule for signal amplification in Listeria monocytogenes detection

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Publication Details

Author list: Oaew S., Charlermroj R., Pattarakankul T., Karoonuthaisiri N.

Publisher: Elsevier

Publication year: 2012

Journal: Biosensors and Bioelectronics (0956-5663)

Volume number: 34

Issue number: 1

Start page: 238

End page: 243

Number of pages: 6

ISSN: 0956-5663

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84858288222&doi=10.1016%2fj.bios.2012.02.011&partnerID=40&md5=a7f58f0b02feb9dc9307c72ffb8093d7

Languages: English-Great Britain (EN-GB)

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Abstract

Bioconjugate nanocapsules were fabricated by using polystyrene sulfonate (PSS) to encapsulate gold nanoparticles (AuNPs) bearing adsorbed horseradish peroxidase (HRP). The average size of nanocapsule was in a range 150-400. nm. The efficiency of the capsules to enhance signals in an immunoassay was demonstrated by using an enzyme linked immunosorbent assay (ELISA) to detect the food-borne pathogen - Listeria monocytogenes. The antibody adsorbed onto the PSS shell of the nanocapsules provided the recognition molecule. For a given quantity of antibody, the bioconjugate nanocapsules showed 30 times greater sensitivity and a shorter assay time (5. min) when compared to conventional ELISA using an HRP labelled antibody. This proof-of-concept encapsulation of HRP through PSS nanocapsules may pave the way for alternative signal enhancement strategies where sensitivity is a priority. ฉ 2012 Elsevier B.V.

Keywords

Listeria monocytogenes, Nanocapsule, Polyelectrolyte