Construction of synthetic escherichia coli producing s-linalool
Conference proceedings article
Authors/Editors
Strategic Research Themes
No matching items found.
Publication Details
Author list: Thanasomboon R., Waraho D., Cheevadhanarak S., Meechai A.
Publisher: Elsevier
Publication year: 2012
Volume number: 11
Start page: 88
End page: 95
Number of pages: 8
ISSN: 1877-0509
Languages: English-Great Britain (EN-GB)
View in Web of Science | View on publisher site | View citing articles in Web of Science
Abstract
Floral scents are used in many industries and demand for them increases every year. However, the shortage of extracted fragrance supplies from plants leads to high market costs of floral scents. Production of these fragrances from microorganisms is an alternative. In this work we applied the synthetic biology approach to construct a synthetic Escherichia coli system to produce linalool, which is the main component in floral scents. The E. coli system was synthesized in a laboratory by constructing 2 new BioBrick parts, GPPS and LIS Bricks. They were employed to construct GPPS and LIS generator devices by combing them with existing BioBricks from the Registry of BioBrick Standards; i.e., BBa R0040, BBa B0030, BBa B0015. The synthetic E. coli system was created by transforming the generators into a nonscent E. coli YYC912 chassis. The system was tested by culturing synthetic cells in LB medium containing kanamycin and tetramycin. It was found that the synthetic E. coli successfully synthesized linalool and exhibited the strongest linalool scent at 96 hours of cultivation. This synthetic cell will be further used as a base strain for future construction of strains that can make higher linalool products for use on the industry level. ฉ 2012 Published by Elsevier Ltd.
Keywords
BioBrick, Escherichia coli, Synthetic biology
Contact Information