Selective determination of homocysteine levels in human plasma using a silver nanoparticle-based colorimetric assay

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Author listLeesutthiphonchai W., Dungchai W., Siangproh W., Ngamrojnavanich N., Chailapakul O.

PublisherElsevier

Publication year2011

JournalTalanta: The International Journal of Pure and Applied Analytical Chemistry (0039-9140)

Volume number85

Issue number2

Start page870

End page876

Number of pages7

ISSN0039-9140

eISSN1873-3573

URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-79959983479&doi=10.1016%2fj.talanta.2011.04.041&partnerID=40&md5=0663014474ab58c31685efe91009e5eb

LanguagesEnglish-Great Britain (EN-GB)


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Abstract

The first use of silver nanoparticles (AgNPs) for the rapid, simple, and selective determination of homocysteine (Hcy) levels in human plasma was studied. Hcy and five other amino acids, including cysteine (Cys), could be distinguished by their different aggregation kinetics, which caused a change in the visible color and a shift in the UV-vis absorption spectra. The difference in the cross-linking (aggregation) rate between Hcy and Cys was used as the basis for developing a selective probe for Hcy and allowed the detection of Hcy in the linear range of 2-12 μM (R2 = 0.9936). The limits of detection and quantification were found to be 0.5 μM and 1.7 μM, respectively. To investigate its selectivity and potential applicability, this AgNP-based method was successfully applied for the determination of Hcy levels in actual biological (human plasma) samples, where the determined levels of Hcy were within the error range of the measured level using the traditional chemiluminescence microparticle immunoassay (CMIA). Thus, the use of AgNPs is a feasible and potentially reliable method for the determination of Hcy levels in biological samples. © 2011 Elsevier B.V.


Keywords

HomocysteineHuman plasmaSilver nanoparticle-based colorimetric assay


Last updated on 2023-28-09 at 07:35