Cellulosibacter alkalithermophilus gen. nov., sp. nov., an anaerobic alkalithermophilic, cellulolytic-xylanolytic bacterium isolated from soil of a coconut garden

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Author list: Watthanalamloet A., Tachaapaikoon C., Lee Y.S., Kosugi A., Mori Y., Tanasupawat S., Kyu K.L., Ratanakhanokchai K.

Publisher: Microbiology Society

Publication year: 2012

Journal: International Journal of Systematic and Evolutionary Microbiology (1466-5026)

Volume number: 62

Issue number: 10

Start page: 2330

End page: 2335

Number of pages: 6

ISSN: 1466-5026

eISSN: 1466-5034

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867177730&doi=10.1099%2fijs.0.027854-0&partnerID=40&md5=edaa31df09f6011c29a33653c600431f

Languages: English-Great Britain (EN-GB)

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Abstract

An obligately anaerobic, cellulolytic-xylanolytic bacterium, designated strain A6T, was isolated from soil of a coconut garden in the Bangkuntien district of Bangkok, Thailand. The strain was Gram-stain positive, catalase-negative, endospore-forming, motile and rod-shaped with a cell size of 0.2-0.3×2.0-3.0 μm. Optimal growth of strain A6T occurred at pH55 °C 9.5, 55 °C. Strain A6T fermented various carbohydrates, and the end products from the fermentation of cellobiose were acetate, ethanol, propionate and a small amount of butyrate. The major cellular fatty acids were iso-C14: 0 3-OH, iso-C15: 0, iso-C16: 0 and C16: 0. The cell-wall peptidoglycan contained mesodiaminopimelic acid. No respiratory quinones were detected. The DNA G+C content was 30.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain represented a new phyletic sublineage within the family Clostridiaceae, with,93.0% 16S rRNA gene sequence similarity to recognized species of this family. On the basis of phenotypic, genotypic and physiological evidence, strain A6T represents a novel species of a new genus, for which the name Cellulosibacter alkalithermophilus gen. nov., sp. nov. is proposed. The type strain of the type species is A6T (=TISTR 1915T=KCTC 5874T). © G 2012 IUMS.

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