Molecular analysis of Helicobacter pylori virulent-associated genes in hepatobiliary patients

บทความในวารสาร

ผู้เขียน/บรรณาธิการ

บัณฑิต คำภูษา

กลุ่มสาขาการวิจัยเชิงกลยุทธ์

ไม่พบข้อมูลที่เกี่ยวข้อง

รายละเอียดสำหรับงานพิมพ์

รายชื่อผู้แต่ง: Boonyanugomol W., Chomvarin C., Sripa B., Chau-In S., Pugkhem A., Namwat W., Wongboot W., Khampoosa B.

ผู้เผยแพร่: Blackwell Publishing Ltd

ปีที่เผยแพร่ (ค.ศ.): 2012

Volume number: 14

Issue number: 11

หน้าแรก: 754

หน้าสุดท้าย: 763

จำนวนหน้า: 10

นอก: 1365-182X

eISSN: 1365-182X

URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867503528&doi=10.1111%2fj.1477-2574.2012.00533.x&partnerID=40&md5=e97babd62df9c0d472aba70b67a82f9a

ภาษา: English-Great Britain (EN-GB)

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บทคัดย่อ

Objectives: The Helicobacter pylori virulence-associated genes in hepatobiliary patients, including vacA, iceA, babA2, cagA and cagE, have not been reported. The aim of this study was to investigate these genes and the association of those and the clinical outcomes in hepatobiliary diseases. Methods: Eighty H. pylori-PCR-positive cases were obtained from hepatobiliary patients, representing both cholangiocarcinoma (CCA) (n= 58) and cholelithiasis (n= 22). The diversity of virulence genes was examined by polymerase chain reaction and DNA sequencing. Phylogenetic analysis of cagA was determined using the maximum parsimony method. Results: The vacAs1a + c/m1, iceA1 and babA2 genes were the most predominant genotypes in both CCA and cholelithiasis patients. The cagA and cagE genes were found significantly more frequently in patients with CCA than those with cholelithiasis (P < 0.05). The cagA positive samples were the Western-type cagA and showed that almost all of the detected sequences in Thai hepatobiliary and Thai gastric cancer patients were classified in the same cluster but separated from the cluster of Japan and other countries. Conclusions: The cagA and cagE genes may be associated in the pathogenesis of hepatobiliary diseases, especially of CCA. Besides the bacterial variation, other host factors may be involved in the pathogenesis of hepatobiliary cancer. ฉ 2012 International Hepato-Pancreato-Biliary Association.

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