Identification of Acetobacter strains isolated in Thailand based on the phenotypic, chemotaxonomic, and molecular characterizations
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Publication Details
Author list: Kommanee J., Tanasupawat S., Yukphan P., Thongchul N., Moonmangmee D., Yamada Y.
Publication year: 2012
Journal: ScienceAsia (1513-1874)
Volume number: 38
Issue number: 1
Start page: 44
End page: 53
Number of pages: 10
ISSN: 1513-1874
Languages: English-Great Britain (EN-GB)
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Abstract
The restriction analysis of 16S-23S rRNA gene internal transcribed spacer regions (ITS) using TaqI, AluI, HpaII, and AvaII revealed that forty-seven bacterial isolates found in fruits and flowers collected in Thailand belong to the genus Acetobacter. They were identified as follows: Group 1 containing 11 isolates was identified as A. pasteurianus, Group 2 containing nine isolates was identified as A. orientalis, Group 3 containing eight isolates was identified as A. lovaniensis, Group 4 including eight isolates was identified as A. indonesiensis, Group 5 containing three isolates was identified as A. tropicalis, Group 6 containing five isolates was identified as A. ghanensis, and Group 7 containing four isolates was identified as A. orleanensis. The differentiation of Acetobacter species by the 16S-23S rRNA gene ITS restriction analysis was discussed. The phenotypic, chemotaxonomic, and molecular characteristics including 16S rRNA gene sequences supported the above-mentioned identification. All Thai isolates were screened for their alcohol dehydrogenase activity and exhibited an activity of 2.05-7.52 units/mg protein at 30 ฐC. Isolate PHD-23 produced the highest quantity of 1.50% acetic acid (v/v) from 4.0% ethanol (v/v).
Keywords
16S-23S rRNA gene ITS restriction analysis, Acetic acid bacteria, Acetic acid production, Alcohol dehydrogenase
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